In Tpm1.1-transfected MDA-MB-231 cells (human breast cancer cell line) which normally lack endogenous Tpm1 (Houle et al., 2007), DAPK1 overlapped with Tpm1.1 predominantly at the cell edge (Figure 3A), where rigidity sensing activity typically occurs. The gene discussed is DAPK1; the disease is breast carcinoma.