BANF1 and infection: After infection of 3D4/21 cells with AH at an MOI of 200, there was no significant change in the expression of LC3-II and p62 in cells treated with Rap/BAF (p > 0.05), indicating that infection of 3D4/21 cells with AH at a high MOI saturated autophagosome formation, although the expression levels of the LC3-II and p62 proteins in the HCQ treatment group increased significantly (p ≤ 0.05) (Figure 6E).