The effect of the secretome of CAAT isolated from patients with obesity and BC was investigated on the mRNA expression levels of CSC markers, such as CD24 and CD44 as well as EMT markers, such as vimentin and E-cadherin, was investigated in MDA-231 and SUM-149 cells using qRT-PCR. This evidence concerns the gene CD24 and obesity due to melanocortin 4 receptor deficiency.