Wtap-HKO mice fed with NASH diet for 5 weeks also showed higher serum FFAs levels, smaller eWAT, and higher lipolysis rate in the eWAT, which are likely due to the increased protein levels of ADCY3, ADCY4, ADCY6, p-HSL, p-PKA substrate and ATGL (Supplementary Fig. 5a–d). Here, PNPLA2 is linked to metabolic dysfunction-associated steatohepatitis.