As shown in Fig. 2f and Supplementary Fig. 2k, Tnfa, Il1β, iNos, Cd14, Csf1, Ccl2, Ccl3, Ccl5, Ccl22, Cxcl2, Cxcl5, Cxcl10 and Cx3cr1 mRNA levels were significantly increased in Wtap-HKO mice under both normal chow and NASH diet feeding conditions. This evidence concerns the gene CXCL2 and metabolic dysfunction-associated steatohepatitis.