Importantly, qPCR analysis of pancreatic tissue from our patient with CPI-DM showed a 16-fold elevation in CXCL9 when compared with pancreatic tissue from control individuals (ΔCt –6.94 for CPI-DM versus median ΔCt –11.18 for normal), consistent with the scRNA-seq data from the CPI-treated mice. This evidence concerns the gene CXCL9 and isolated cleft palate.