SPINK1 and neoplasm: Except for the expression of SPINK1 not being associated with the stromal score (non-immune cells) and being negatively correlated with tumor purity, the ESTIMATE and immune scores were positively correlated with the abundance of SPINK1 (Figures 8E–H), suggesting that SPINK1 could be considered as an indicator of the unique reflection of a hot or immune cell-infiltrating tumor microenvironment (41), with the likelihood of a positive response to ICB in HCC patients (42).