The calibration curves between the predicted and observed values for the SPINK1-associated models generally fitted well with the predicted probability of 0.3–1.0 along the x-axis compared to the AFP model alone, in which the goodness of fit between the two curves remained better only in the range 0.7–1.0 and exhibited poor calibration particularly within the range of <0.35, where a relatively low incidence rate of predicted HCC occurred, suggesting the poor sensitivity of AFP (Figures 2B–D). This evidence concerns the gene SPINK1 and hepatocellular carcinoma.