Upregulation of Exo hsa_circ_0050334 promoted colorectal cancer progression by activating the Exo hsa_circ_0050334-hsa_miR_182_5p-RGS2 axis, leading to the increased expression level of RGS2. RGS2, a regulator of G-protein signaling 2, possesses an RGS domain that mediates its GTPase protein-activating activity towards G-protein subunits, which activates phospholipase C intracellularly to exert a biological effect. This evidence concerns the gene PITX2 and colorectal cancer.