IGF1 and adenoma: In the FZD9-/- adenoma cell lines compared to the WT cell line, we found increased IGF-1, BCL-W and HSP60, and decreased p53, IGFBP2, and TRAILR2, all supporting that loss of FZD9 contributes to increased cell survival in adenomas (Figure 5B) (Dot blot images are in Supplementary Figure 2).