INS and infection: At the end of the experimental period, animals were humanely sacrificed using CO2 on day 0, 7, 14, 28, 35 and 42 (n = 6 in each group) post Tz-infection for collection of sera and rat carcasses for determination of serum insulin and trichinella adult worm and muscle larvae load respectively (Murambiwa et al., 2020).