To investigate the effect of NETs on atherosclerosis in vivo, we generated neutrophil-specific Pad4 knockout mice (ApoE−/−; Neu-Pad4−/−) by crossing Pad4fl/fl mice with Tg-Mrp8-Cre mice and then crossing them with an ApoE−/−mice. The gene discussed is APOE; the disease is atherosclerosis.