Western blot results showed that stimulation of S1R in HF + F rats promoted phosphorylation of JAK2 and STAT3 markedly (Robson et al. 2014), without any alterations on total JAK2 and STAT3, which further upregulated downstream p-VEGFR2 and VEGF expression (Fig. 4Eand Fig. S3). This evidence concerns the gene TMBIM4 and hydrops fetalis.