To further explore the function of AOC1, we conducted a series of function studies of AOC1. Western blot and qRT-PCR verified that DU145 and 22Rv1 have relatively low expression of AOC1 in all prostate cancer cell lines, but that LNCaP and PC-3 have the higher expression (Fig. 3a, b). The gene discussed is AOC1; the disease is Familial prostate cancer.