To test this hypothesis, a non-pathogenic but cross-reactive anti-Dsg1 autoantibody clone (PF1-2-22), previously isolated from PF patients (Yamagami et al., 2009), was produced as the GASDALIE variant and confirmed to have different binding epitope as pathogenic anti-Dsg1 clones PF1-8-15 and PF24-9 (Figure 4—figure supplement 2D, Supplementary file 1e). This evidence concerns the gene DSG1 and pemphigus foliaceus.