To further investigate whether Fc-FcγR interactions attenuate anti-Dsg1 autoantibody pathogenicity, the N297A and GASDALIE variants (with reduced FcγR binding and enhanced activating FcγR binding, respectively [Figure 1—figure supplement 1B, C; Bournazos et al., 2019; Sazinsky et al., 2008]) of IgG1 anti-Dsg1 autoantibodies were produced (Figure 4—figure supplement 2A) and evaluated in the adult pemphigus model. This evidence concerns the gene DSG1 and pemphigus.