Within the gained regions, ATAD2, MYC, RUNX2, RECQL4, ERBB2, PDGFRA, and CCNE1 were recurrently observed in the tumors and appear to be potential drivers, confirming previous studies in osteosarcoma (Chen et al, 2014; Kovac et al, 2015; Both et al, 2016; Bousquet et al, 2016; Sayles et al, 2019; Suehara et al, 2019; Guimarães et al, 2021). This evidence concerns the gene RUNX2 and osteosarcoma.