LAP+ iTregs exert their suppressive role in a TGF-β-dependent manner [79] and neutralization of these cells by intravenous administration of anti-LAP antibody prevents oral tolerance induction in murine models of experimental autoimmune encephalomyelitis (EAE) [14, 80] and arthritis [81]. This evidence concerns the gene TGFB1 and arthritic joint disease.