However, treatment of Bifico could restore protein expression to normal levels (TNF-ɑ: the IBS group vs. the IBS + Bifico group: P < 0.05, the control group vs. the IBS + Bifico group: P > 0.05, respectively and IL-6: the IBS group vs. the IBS + Bifico group: P < 0.05, the control group vs. the IBS + Bifico group: P > 0.05, respectively) (Fig. 6), which indicated that Bifico treatment relieved the colonic inflammation in IBS mice. This evidence concerns the gene TNF and irritable bowel syndrome.