Infection of cells with enteropathogenic E. coli, as well as overexpression of EspG and VirA (Golgi stress-inducing bacterial toxins from enteropathogenic E. coli and Shigella respectively) also upregulated SIRT2 consistent with a conserved mechanism of SIRT2 regulation during bacteria-induced Golgi stress (Supplementary Fig. 2F-G). Here, SIRT2 is linked to infection.