We verified this by LC-MS analysis of adenosine and immunostaining of CD4, CD39, CD206 and CD73 expression in an orthotopic breast tumour model by inoculating firefly luciferase-transfected 4T1 (fLuc-4T1) tumour cells into the breast pads of BALB/c mice. The gene discussed is ENTPD1; the disease is breast neoplasm.