Flow cytometry analysis of the polarization of tumor-associated macrophages (TAMs) demonstrated CD80+ macrophages greatly improved and CD206+ macrophages significantly decreased in the tumors treated with URB + US in comparison with control, only US and only URB groups, indicating that URB + US treatment could effectively promoted the polarization of TAMs from M2 phenotype towards M1 phenotype (Fig. 5e–h, Supplementary Figs. 9, 10b). Here, CCDC80 is linked to neoplasm.