PTEN and non-small cell lung carcinoma: Besides, the miR-494-3p mimic was subsequently transfected into A549 cells to construct miR-494-3p overexpression model (Fig. 4D), and western blot assay proved that the expression level of PTEN protein in NSCLC cells was decreased significantly after up-regulating miR-494-3p (Fig. 4E).