To further understand the action mechanism of SNHG20 expression in 5-FU drug-resistant colon cancer cells, silence and overexpression SNHG20 slow virus carriers were established, and they were transfected into colon cancer HT29 cells for the downregulation and upregulation of SNHG20 in cells. This evidence concerns the gene SNHG20 and malignant colon neoplasm.