Surprisingly, the HFD had no significant effect on NLRP3 mRNA in sperm, slightly increased the NLRP3 mRNA levels in Leydig cells, and markedly increased the NLRP3 mRNA levels in SCs (Fig. 1C), suggesting that NLRP3 is mainly derived from SCs under obesity conditions. Here, NLRP3 is linked to obesity due to melanocortin 4 receptor deficiency.