Next, to further verify whether SIRT6 regulates glycolysis in NSCLC cells through the HIF-1α/HK2 signaling axis, PX-478 2HCl, a HIF-1α inhibitor, was used to suppress the expression of HIF-1α in SIRT6-overexpressing cells. This evidence concerns the gene HK2 and non-small cell lung carcinoma.