We further performed TUNEL staining to analyze the apoptosis of tumor tissues in each group and found that the proportion of apoptosis in the SIRT6 overexpression group decreased, while the proportion of apoptosis in the SIRT6 interference group increased (Fig. 7E), and the difference in the proportion of positive cells in each group was statistically significant (Fig. 7F), which is consistent with the results of the in vitro experiment. The gene discussed is SIRT6; the disease is neoplasm.