Consequently, we suggest that anxiety-like behavior in the chronic phase may be related to alterations in the Cdkn1a, Ciart, Fos, Hspa5, Hapb1 and Klf10 genes, and additional analysis, including WGCNA, are required to compare RNA-seq data with behavioral dysfunctions (e.g. memory impairment, depression, and anxiety) in a variety of brain regions in irradiated mice. Here, HSPA5 is linked to major depressive disorder.