As a positive control to show that the MFF S146 site is regulated by AMPK activation, we overexpressed the human isoform of MFF in Neuro2A (N2A) neuroblastoma cells and treated with Metformin, a potent activator of AMPK40, or a vehicle control (Supplementary Fig. S5a, b). The gene discussed is PRKAA1; the disease is neuroblastoma.