TGFBR2 and breast carcinoma: The TβRII ELISA gave similar results to FACS assay: ROC curves indicated that circulating EV-TβRII protein shows an AUC of 0.967 a sensitivity of 89.13%, a specificity of 100%, an accuracy of 92.42%, and positive and negative predictor values of 100% and 80%, respectively (Fig. 3f and Supplementary Table 3), supporting its utility as a biomarker for breast cancer and its potential for early detection of metastasis.