In correlation with liver fibrosis as one of the major pathways enriched among DEGs in obese and palmitate treated iPSCs, we found several genes of the TGFβ signaling pathway also differentially expressed (Fig. 5A and Supplementary files 1–16) mostly on day 13 and day 17 of hepatic differentiation. This evidence concerns the gene TGFB1 and Hepatic fibrosis.