For example, genes, BMPR1B, TGFB2, TGFB1I1, SMAD3, NUAK1, and NUAK2 were downregulated in obese iPSCs in both control and palmitate treated cells compared to normal iPSCs also showed downregulation to varying degrees in NAFLD samples (Fig. S2B). The gene discussed is NUAK2; the disease is metabolic dysfunction-associated steatotic liver disease.