We therefore measured cleaved-Caspase 8, cleaved-Caspase 3, phosphorylated RIP1, phosphorylated RIP3 and phosphorylated MLKL in mucinous and non-mucinous CRC cell lines treated with 25 μM 5-FU or DMSO control by western blot (Figure 4 and Supplemental Table 3). The gene discussed is MLKL; the disease is colorectal carcinoma.