The results indicated that the expression level of AC025754.2, AL451165.2, and AL513550.1 were significantly increased in ESCC tissue compared with normal adjacent tissue, whereas HAND2-AS1, SNHG7, SRP14-AS1, and AC007566.1 were downregulated in ESCC tissue (Figure 10A). The gene discussed is HAND2; the disease is esophageal squamous cell carcinoma.