To further validate necroptosis-related genes expression in HCC, IHC was used to measure the expression level of necroptosis-related genes in HCC (N=8), and the result showed that compared with normal group, the CASP8, CDKN2A, DNMT1, EZH2, HSP90AA1, HSPA4, MYCN, PLK1, SLC39A7, SQSTM1, TNFRSF21, TRAF2, TRIM11, and USP22 level were significantly higher in HCC group, while ALDH2 was downregulated in HCC versus normal tissues (Figures 10A, B). The gene discussed is TNFRSF21; the disease is hepatocellular carcinoma.