In a previous study by Rujito et al. (12) regarding the genotype distribution of beta-thalassemia in the Javanese population of the south region of Central Java, in 209 patients, genotypes were as follows: CD26 /IVSI-5 (40.67%), followed by IVS-I-5/IVS-I-5 (14.83%). The gene discussed is DPP4; the disease is beta thalassemia.