ICAM1 and breast neoplasm: To assess the effects of similar circulating TENs on the metastatic potential of either control or ICAM-1 KO E0771 cells in our experimental system, we designed a protocol for the i.v. injection of control E0771 cells to syngeneic recipient mice followed by a transfer of spleen cell suspensions from control E0771 breast tumor–bearing donor mice to the recipient mice (Figure 7A).