Among 227 lactate metabolism-related genes, 37 DEGs in COAD patients of TCGA cohort were identified with FDR< 0.05 and logFC >1, 27 of which were up-regulated (TRMU, TIMM50, POLG2, CHEK2, CFI, CARS2, LDHB, GTPBP3, NDUFAF8, IRAK1, PNPT1, CLPB, CD46, ATAD3A, PDP1, COL4A1, POMK, SLC16A8, HPDL, TWNK, PUS1, KCNN4, MYC, CA5A, HS6ST2, SPP1 and SLC13A3) and 10 were down-regulated (SLC5A12, LDHD, HBB, ACAT1, ACADM, SLC25A42, FLI1, LARGE1, CFH and MPC1). This evidence concerns the gene NDUFAF8 and colon adenocarcinoma.