To investigate to what extent IGF1 also creates an anti-inflammatory phenotype in neutrophils and macrophages in vivo we exposed mice to MI followed by reperfusion with and without IGF1 treatment and performed single cell RNA-sequencing (scRNA-seq) of cardiac myeloid cells, 3 days post-MI (Figure 6A). The gene discussed is IGF1; the disease is myocardial infarction.