Here we have shown that the EnzChekTM substrate can be used to quantify LPL activity in bLPL, (Figure 5A), LPL enriched HR fractions from BV-2 microglia, but not in HF fractions from BV-2 microglia where LPL expression has been knocked out using CRISPR-Cas9 (Figure 5B), and PHP (Figure 5C). Here, LPL is linked to hydrops fetalis.