To find the possible involvement of PI3K/AKT pathway in ESCC cells after COQ10B silencing, the assessment of western blot revealed a significant reduced expression of AKT and p-AKT protein expression in LV-shCOQ10B group as compared to the LV-shCtrl group (Figure 8), revealing the regulation role of COQ10B in KYSE-150 cells via PI3K/AKT signaling pathway. The gene discussed is AKT1; the disease is esophageal squamous cell carcinoma.