As shown in Figure 5B-5E, the total number and cell body (i.e., soma) area of ChAT+ MNs were significantly decreased in both 19 wk male and female hSOD1G93A naïve mice compared to WT mice, consistent with previous work that showed ALS pathology preferentially affects larger alpha motor neurons innervating faster muscle fibers 36. The gene discussed is CHAT; the disease is amyotrophic lateral sclerosis.