In addition, the release of DAMPs obviously caused DCs maturation, subsequent tumor infiltration of CD8+ T cells and decline of regulatory T cells (Tregs), which resulted in the highest content of mature DCs, CD8+ T cells and minimum expression of Tregs in the OPCPN@NTKPEG (+) group compared to other groups (Figure 3E-G), and eventually realized the best antitumor immunotherapeutic effect in PDIT. This evidence concerns the gene CD8A and neoplasm.