To further verify that the metastatic phenotype identification of the tumors is driven by the changes in the tumor microenvironment that result from alterations in gene expression, we compared the MCR-ALS scores of the Raman spectra obtained from the TWIST-KO, FOXC2-KD, and CXCR3-KD tumors with their respective controls. Here, TWIST1 is linked to neoplasm.