Kaplan-Meier analysis of the top 3 significantly overexpressed splicing factors demonstrated that while YBX1 and PTBP1 did not show prognosis differences (Figure S1D), high expressing NONO tumors (LGG and GBM) exhibited shorter overall survival (OS) compared with low expressing NONO tumors based on the CGGA data (Figure S1E). Here, PTBP1 is linked to glioblastoma.