Zhao et al. (2021) confirmed that the probiotic mixture (B. infantis, L. acidophilus, Enterococcus, and Bacillus cereus) could enhance the expression of claudin-1 and occludin, by regulating the pregnane X receptor-c-Jun N-terminal kinase signaling pathway, and ameliorate intestinal barrier damage in neonatal necrotizing enterocolitis. Furthermore, co-culture of Saccharomyces boulardii CNCM I-745 with T84 cells in vitro revealed that S. boulardii altered the distribution of ZO1 and maintained the barrier function (Pontier-Bres et al., 2015). The gene discussed is OCLN; the disease is necrotizing enterocolitis.