KEGG pathway enrichment analysis indicated that hub genes were remarkably enriched in bladder cancer, endocrine resistance, proteoglycans in cancer and pathways in cancers, such as PI3K-Akt signaling pathway, ErbB signaling pathway, VEGF signaling pathway and MAPK signaling pathway (Figure 5D and Supplementary Table S3). The gene discussed is EGFR; the disease is urinary bladder carcinoma.