In detail, TMEM154, TSPAN3, CAMK1D, TRHDE, PEG10, ME1, SATB2, SNAP25, ANKRD18A, ISM1, and SGCB were upregulated in NAFLD, while APOF, SYP, OPN3, CENPV, IGF1, AMDHD1, P4HA1, and MRPL21 were downregulated within NAFLD samples (Figure 1D). This evidence concerns the gene TSPAN3 and metabolic dysfunction-associated steatotic liver disease.