TNF and melanoma: Additionally, intracellular cytokine phenotyping of TIL-B within single-cell suspensions obtained from melanoma lesions evaluated by flow cytometry showed no significant differences in the low percentages of IL-10-expressing Bregs among CD19 + B cells in the TME compared to the patient circulation, while TNF-α-expressing (CD19+) B cells were significantly reduced in melanoma lesions compared to the circulation (Supplementary Figure 7(a-c)).