Although there were no significant differences in overall expression of TGF-β, IL-10, or TNF-α between melanoma lesions and normal skin (bulk RNA-seq, Supplementary Figure 5), IHC/IF evaluations identified cytokine-expressing TIL-B, found in close proximity to CD3+ TIL-T (Figure 3(f-g), Supplementary Figure 6). Here, TGFB1 is linked to melanoma.