CD27 and melanoma: CyTOF analyses of peripheral blood B cells (CyTOF 34-marker panel) identified enriched circulating TGF-β and PD-L1-expressing CD19+ CD38int IgD+ CD27− Bregs in melanoma patients (N = 26) compared to age- and sex-matched HV (N = 12) (Figure 1(g-h)).