The expression level of PI3K/Akt pathway-related proteins in HCC cells in the circFOXO3 mimic + PI3K activator group was significantly higher than that in the circFOXO3 mimic group, suggesting that the overexpression level of circFOXO3 could inhibit the proliferation and migration of HCC cells by inhibiting the PI3K/Akt pathway. This evidence concerns the gene AKT1 and hepatocellular carcinoma.