To determine whether the necab2 mutant displayed altered behaviors as those commonly observed in ASD patients, we recorded larval locomotion and anxiety-associated behaviors, adapted as markers of ASD behaviors in zebrafish (Kozol et al., 2015; Zimmermann et al., 2015; Li et al., 2019; Maphanga et al., 2020). The gene discussed is NECAB2; the disease is Anxiety.