In our study, the role of ADAM17 knockdown in AMPK/mTOR pathway and its downstream p-4EBP1 and p-p70S6K were examined in differentiated H9c2 cells, which showed that cardiomyocyte-specific knockout of ADAM17 virtually reversed the inhibition of AMPK phosphorylation in DCM and ADAM17 played an important role in regulating AMPK/mTOR signal pathway. This evidence concerns the gene ADAM17 and familial dilated cardiomyopathy.