CD80 and neoplasm: Hyperthermia significantly increases the proportion of mature DC subtypes (CD11c+, CD80+, CD86+).Hyperthermia stimulates the differentiation of naive T cells to CD8+ T cells.Hyperthermia significantly improves the tumor infiltration of CTL.Hyperthermia decreases the levels of CD4, CD25, and Foxp3 in Tregs and reduces the number of MDSC.Hyperthermia increases the expression of IFN‐γ and TNF‐α.