To further demonstrate the correlation between cancer stemness and IFN signalling activation, we isolated CD44High and CD44Low DU145 populations, and observed an increase in stemness properties along with higher level of JAK1, STAT1, IFIT5, BMI1, Nanog and SOX2 proteins expressing in CD44High DU145 cells, when compared to CD44Low DU145 subpopulation (Figure S5E). This evidence concerns the gene IFNA1 and cancer.