Thus, we re-clustered CD4 Tregs and further identified four states of Tregs, including IL2RA-high and CCL22 positive subtype (IL2RAhi-CCL22+-Treg), IL2RA-high subtype (IL2RAhi-Treg), Type 1 IFN positive subtype (Type1IFN-Treg), and IL2RA-Low subtype (IL2RAlo-Treg) (Fig. 5f), together consisting 34.3% of the tumor-infiltrating CD4+ T cells (Fig. 5c). The gene discussed is CCL22; the disease is neoplasm.